Category Archives: Research

Pose and Click: Hassle-free goat sampling in Ethiopia

Sarah Osama takes blood samples from a goat in Ethiopia

Sarah Osama takes blood samples from a goat in Ethiopia

Narrated by Sara Osama, Research Technician

Taking blood, tissue or hair samples for genetic analysis and at the same time doing physical measurements of livestock in the field can be a very hectic and time consuming activity.

This tedious but necessary sampling process has greatly been eased by the use of a sampling method (AdaptMap photo protocol and sampling kit) developed by United States Department of Agriculture (USDA). When I visited the villages of Haro Wolkite and Luma Tatesain in Ethiopia on a mission to investigate the genetic basis of goats in that region, I had the opportunity to test the sampling kit which had a slight modification made to it at the Hub.

Photo shoot
By taking photographs of the goats at different angles, we were able to deduce the physical measurements of the goats without struggling to pin them down. The process involved making markings on the animal’s pin bones which could easily be viewed by the photographer. An identifying card was then attached to the animal on which information such as the animal’s unique identity number, sex, birth date, owner, breed, sampling date, district/location, country and the distance from the camera was recorded.

A photograph of the goat’s eye with a color guide placed next to it was taken to determine the anemic state for the animal. This “famacha” guide has five levels of red and an animal giving a low score of 5 (very pale red) indicates the animal could be suffering from anemia. A photograph of the teeth helped us estimate its age while various profile shots were taken to deduce the pin bone width; the chest girth; height and length; and the points of shoulder width. All the information acquired from the photo shoot was recorded onto the card attached to the animal.
Finally, blood samples are drawn immediately after taking photos of each animal. The blood samples were labeled using the identity numbers given to the animals while taking the pictures for physical measurements.

Take and give
The farmers in the two villages visited were very cooperative with the researchers and gave extra assistance in managing the blood samples on FTA cards (cards developed for the collection and storage of DNA from organic samples), as they dried under the shade of a tree before storage. The farmers expressed their satisfaction with the minimal handling of their animals and in return for their cooperation in the sampling process they received on-the-spot diagnosis and treatment for worms based on the eye exam. Since the team included production experts, the farmers also received advice on better production practices.

This sampling exercise was part of the field activities being carried out by the BecA-ILRI Hub led team researching the genetic diversity of goats for improved productivity in Ethiopia and Cameroon.

Coming to Africa: Q&A with Dr Ghimire

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Dr. Sita Ghimire joined the BecA-ILRI Hub in June 2013 as senior scientist for the Swedish funded “Climate-smart Brachiaria grasses for improving livestock production in East Africa” program. Prior to joining the Hub, Sita was a research microbiologist at the Research Triangle Institute (RTI) International in North Carolina, USA.

Find out more about his passion for his work and what drew him out of USA and into Africa in this article.

Q Dr Ghimire, in your online profile, you talk about growing up in a farming family in Nepal. Did your early family life have anything to do with your choice of career?

A When I was growing up, almost 95% of the population in Nepal depended on agriculture for their livelihoods. As a farming community, we all looked up to government employed agricultural officials to solve all our farming problems. They were very highly regarded in the community and it was every child’s dream to graduate from high school, be trained in an agriculture college and become an agricultural official. That was my ultimate dream too. However after joining the agriculture school, my eyes were opened up to so many other possibilities beyond being a local extension official.

Q Can you tell us a bit about your journey from extension official to crop research scientist?

A After my undergraduate studies, I worked in remote villages where people’s livelihoods were based on potatoes. My role there was to support a community based potato bacterial wilt management program funded by the User’s Perspective with Agricultural Research & Development Program, a sister organization of International Potato Center (CIP). The area had a severe bacteria wilt problem and the idea of the program was to alleviate potato losses through the implementation of an integrated disease management approach that included a three year crop rotation with non-Solanaceous crops. The plant pests and diseases problems faced by these farming communities persuaded me to specialize in plant pathology for my Masters’ degree, and later conduct research on potato late blight pathogen as part of my PhD studies.

Q How did you end up in the USA?

A During a period of civil unrest in Nepal (1996-2006), government funding on agricultural research and development was severely affected. Law and order in the country was also deteriorating and as a result many people from Nepal moved to other parts of the world. I moved to USA in 2003, taking up a Post-Doctoral position with the Mississippi State University.

Q Your stay in the US ended up being more than 10 years – what made you choose to come to Africa?

A My greatest desire at the time I responded to the job advertisement was to move from working in a commercial environment, to doing research that I was sure will have an impact for millions of small holder subsistence farmers of sub-Saharan Africa. I was sure I would get more satisfaction from the kind of impact I could have in such a position. Something else that touched me was the fact that Appolinaire, the Director of the BecA-ILRI Hub left his job as an assistant professor in a very prestigious institution in the US to come and work here. I thought – if he could, why couldn’t I?

Q What excites you most about this research program?

A There are so many things that make my work exciting! Over the past several decades, extensive research has been carried out on endophytes (beneficial microorganisms growing within the plants) of cool season grasses, the grasses mostly grown in temperate parts of the world. However, the endophytes of warm season grasses, grasses commonly found in the tropics including Brachiaria are very little researched. This program provides the unique opportunity of studying Brachiaria grasses and their associated microbes in their center of origin – East Africa.

In addition, through this program I see a big opportunity to take the ground-breaking research of Dr Segenet Kelemu (former BecA-ILRI Hub director) and her colleagues to the next level and make it benefit farmers – there is a possibility of developing microbe based pesticides, herbicides and fertilizers with wider applications.

Lastly, since the BecA-ILRI Hub has such a broad mandate to work in eastern and central Africa and even beyond, I look forward to the prospects of developing the Hub into the leading endophyte research center in Africa. I am very hopeful about the future.

 

Toxic scourge – A sub-Saharan African perspective of aflatoxin contamination

Benoit Gnonlonfin

Benoit Gnonlonfin in a maize farm in eastern Kenya

For 15 years, Benoit Gnonlonfin has dedicated himself to the study of mycotoxins and especially aflatoxin contamination of food crops in sub-Saharan Africa.

Aflatoxins (Aspergillus mycotoxins) are estimated to contaminate 25% of the global food supply – with 4.5 billion people exposed to high, unmonitored levels – primarily in developing countries. These highly carcinogenic fungi by-products are associated with immunosuppression, reduced nutrient absorption and stunting of infants.

Not much is known about the level of aflatoxin contamination of feed and foodstuffs in Africa, yet it causes untold economic losses to small holder farmers, the majority food producers on the continent.

“My dream is to help these farmers who have little or no technology to prevent their crops from being contaminated” says Benoit.

Benoit’s passion in this area of research has led him to write numerous articles on mycotoxins, 22 of which have been published in international peer-reviewed journals. His latest article published in the Critical Reviews in Food Science and Nutrition Journal highlights research questions on the conditions conducive to aflatoxin production, contamination processes and evaluates the potential risk of exposure from common African foods.

The publication of this article coincides with a critical phase of the BecA-ILRI Hub led “Capacity and Action for Aflatoxin Reduction in Eastern Africa (CAAREA)” project that Benoit is involved in which is looking at adapting aflatoxin diagnostics and analysis tools to the African small holder farmer’s context. As part of the CAAREA team, Benoit has played a major role in establishing a state-of-the-art regional mycotoxin diagnostic platform which is already in use by universities and other partners including advanced research institutions conducting mycotoxin-related research projects.

Benoit has dedicated many years of his career to investigating these issues. In response to the question on the demands his research and writing has placed on him, Benoit says:

“When you have such an important objective, to improve the level of food safety and security in Africa, you must make a lot of sacrifices to accomplish it”.

Read the full review article here .
Read more about the AusAID funded CAAREA project here .

Mushrooming interest in edible indigenous fungi

Media coverage received by the BecA-ILRI Hub led “Wild edible mushrooms: exploring opportunities for domestication of species in eastern Africa ” project has contributed to increased awareness and participation by local communities in Burundi. Members of the press from Radio Isanganiro , private broadcaster Tele Renaissance and state-owned broadcasting Radio Télévision Nationale du Burundi were present during stakeholder workshops held by the project team in October and November 2012 in Bujumbura. Continue reading