Investigating the role of bushmeat in the transmission of zoonotic diseases in Tanzania

Research conducted by the Biosciences eastern and central Africa-International Livestock Research Institute (BecA-ILRI) Hub in collaboration with National Health Laboratory of the Tanzania Ministry of Health and Social Welfare; Nelson Mandela African Institution of Science and Technology (NM-AIST); Sokoine University of Agriculture; Tanzania National Parks; Tanzania Wildlife Research Institute; Frankfurt Zoological Society; and Pennsylvania State University.

An outcome of the BecA-ILRI Hub’s Swedish funded initiative to strengthen infrastructural and human capability at NM-AIST, was the awarding of a grant to the institution by the US Defense Threat Reduction Agency.

The NM-AIST School of Life Sciences and Bioengineering and a consortium of partners including the BecA-ILRI Hub received a grant to investigate the role of bushmeat in the transmission of six pathogens between animals and humans in Tanzania.

An interdisciplinary and multi-institutional team of scientists from Tanzania, Kenya and the US are using state-of-the-art techniques to map the distribution of anthrax, ebola, marburg and monkeypox viruses as well as Brucella and Coxiella in bushmeat in Tanzania. The team assesses the biological risk and potential for impact on human health from these diseases.

The BecA-ILRI Hub provides capacity building, expertise and technology for the microbiome component of the project using the genomics platform. During a week-long workshop facilitated by the BecA-ILRI Hub at NM-AIST, Francesca Stomeo provided training on the theory and practice of the genomics pipeline to be used in the project.

Read more about the bioscience research and innovations that underpin development outcomes in the BecA-ILRI Hub 2016 Annual Report.

Research facilitated by the BecA-ILRI Hub drives the bioscience innovations that underpin development outcomes. The success of the climate-smart Brachiaria program in developing technologies that are readily adapted by farmers has generated a demand for their scaling-up. Strategic partnerships, for instance with the North Carolina State University (NCSU)—leveraging the human resource of advanced research institutions—have enabled groundbreaking work in tackling the devastating cassava mosaic disease, potentially increasing yields in what is a staple crop for over 250 million people in Africa.

Through the Africa Biosciences Challenge Fund (ABCF) fellowship program, up and coming research fellows from national agricultural research systems (NARS) have contributed to the formulation of evidence-based agricultural policies. For instance, seminal work on maize and food safety has provided a clearer picture of the interventions required throughout the value chain in Rwanda and research into chicken vaccines has supported the elaboration of policies guiding the production of vaccines for infectious bursal disease in Kenya. Moreover, the establishment and support of communities of practice (CoPs) for ABCF alumni has enabled the development of a comprehensive regional approach to the tackling of key livestock and crop research challenges.

In step with technology advances, the BecA-ILRI Hub launched the Integrated Genotyping Service and Support (IGSS) platform to enhance efficiency and precision in plant and livestock breeding, as well as quality seeds assessment. In research led by the International Maize and Wheat Improvement Center (CIMMYT), application of this new technology has improved understanding of the genetic basis for resistance to maize lethal necrosis (MLN). The ongoing upgrading of the BecA-ILRI Hub’s technology platforms is fast-tracking research within the regional NARS and reducing the need for scientists to leave Africa to do their work.

Working to shape to continent-wide processes, BecA-ILRI Hub staff joined CGIAR research scientists, policymakers, and representatives of higher education networks and the private sector at a workshop to develop the concept of the the African Agricultural Research Programme (AARP). AARP is an initiative led by the Forum for Agricultural Research in Africa (FARA) to strengthen the continent’s agricultural research systems for increased productivity, profitability and sustainability. As part of our 2018–2023 strategy, the BecA-ILRI Hub will seek to play a leading role in the application of and support for biosciences in the region. A landscape survey confirmed the comparative advantage of the BecA-ILRI Hub as an important regionally-valued bioscience facility. It identified opportunities to enhance our role in helping set the bioscience agricultural research agenda, as well as an advocate for the government funding of NARS work in bioscience technologies and services.

The coming year will, therefore, be characterized by engagement with key stakeholders to guide the development of our new five-year plan. We remain committed to helping Africa use biosciences as a means of transforming agriculture, bridging the gap between population growth and agricultural productivity on the continent. To the readers of this report, we hope you will accompany us on this grand AR4D journey in Africa. To our many partners and donors, thank you for your support.

Jimmy Smith
Director General, ILRI

Appolinaire Djikeng
Director, BecA-ILRI Hub

You can download the full 2016 Annual report: http://hdl.handle.net/10568/83016

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Aphids, leafhoppers and whiteflies are responsible for the spread of diseases causing significant crop yield losses globally. On 5 July 2017, the Biosciences eastern and central Africa-International Livestock Research Institute (BecA-ILRI) Hub hosted a symposium to explore ways in which the knowledge of plants, disease-causing organisms and their vectors can be used to combat devastating crop diseases in Africa.

Stephen Runo of Kenyatta University (left) with JIC scientists Beccy Corkill, Olu Shorinola and Sam Mugford (photo JIC/Matt Heaton)

In sub Saharan Africa, the aphid-transmitted bean viruses—bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV)—cause up to 100 percent losses for smallholder bean farmers. Growers of cassava—a staple food for over 250 million people— experience losses of up to 23 million tonnes annually across Africa due to disease caused by whitefly-transmitted Cassava mosaic viruses.

In the face of increased regulations on the use of pesticides, a better understanding of the plant-microbe-vector interactions could lead to the development of urgently needed bio pest-controls. The July forum brought together researchers from the BecA-ILRI Hub, Kenyatta University, International Institute of Tropical Agriculture (IITA), Auburn University and North Carolina State University based in Africa; and the John Innes Centre (JIC) from UK.

From left to right: Josiah Mutuku (BecA-ILRI Hub), Olu Shorinola (JIC), Steven Runo (Kenyatta University), Beccy Corkill (JIC) and Sam Mugford (JIC) at the BecA-ILRI Hub greenhouses (photo: JIC/ Matt Heaton

The symposium was held under the Alliance for Accelerated Crop Improvement in Africa (ACACIA) initiative—a new initiative established to harness diverse research efforts for hastened crop improvement in Africa.

Read full story: Deciphering Plant-Insect Interactions on the ACACIA website.

Read about the ACACIA initiative: New initiative to accelerate crop improvement for food security in Africa

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Goat in a market in Nigeria (photo credit: ILRI/Mann).

From 19–30 June 2017, the Biosciences eastern and central Africa-International Livestock Research Institute (BecA-ILRI) Hub will host the third edition of the Animal Quantitative Genetics and Genomics annual training workshop. The training is strengthening the capacity of researchers in Africa to apply an in-depth understanding of livestock genetics to the design of livestock breeding programmes.

Early this month (8–12 May 2017) over 250 experts from the public and private sectors in more than 50 countries across the globe gathered in Addis Ababa, Ethiopia to discuss the benefits and potential of livestock during the Global Agenda for Sustainable Livestock (GASL). The increasing demand for animal protein in emerging economies in Africa presents the challenge of sustainably improving livestock productivity while at the same time maintaining genetic diversity.

Since 2012, the BecA-ILRI Hub has been conducting research to improve performance of indigenous goats using their genetic diversity. Working in Cameroon and Ethiopia, the “Harnessing genetic diversity for improved goat productivity” project looked at the genetic adaptation of goat populations in the two countries to environmental challenges including drought and disease.

To Getinet Mekuriaw, an assistant professor at Bahir Dar University in Ethiopia and a visiting scientist at the BecA-ILRI Hub, the key to sustainable development of livestock in Africa is in the optimal exploitation of genetic resources to improve indigenous breeds.

‘We have the evidence of a rich genetic resource in livestock in Africa, and particularly in indigenous goats,’ Mekuriaw said ‘the next step is investing in research that will link this intelligence to the design of trait-focused breeding programs.’

Mekuriaw’s PhD contributed largely to establishing the extent of diversity among indigenous goat breeds in the two countries of interest for the BecA-led research. He also investigated the genetic potential of the goat populations in adaptation, disease resistance, reproduction and hair fibre production.

Strategies to enhance livestock production–including exploiting the natural potential of local breeds–could greatly contribute to the realization of the 2030 Agenda for Sustainable Development through increased agricultural capacity in developing countries.

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Read more about the 7th Multi-stakeholder partnership meeting of the Global Agenda for Sustainable Livestock

Read related post – Cooperating with the future: Towards multiplying the multiple benefits of sustainable livestock 

Fred Masika at UC Davis, US during the 13th Solanaceae Conference September 12— 16, 2016

The modernization of agriculture in Africa has led to the focus on cultivation of a very limited variety of food crops. Sadly, this means we are missing out on nutritional and health benefits found in traditional plants such as the African eggplant.

The African eggplant (Solanum aethiopicum) is not only a vegetable, but also has medicinal value. Skin ailments, asthma, bronchitis, diabetes and blood cholesterol are some of the health disorders that this plant is known to alleviate. In Uganda, the local variety ‘Nakati’  is increasingly gaining importance as a source of income and nutrition for smallholder farmers, mostly women and youths. I want to contribute to research that will boost its production and enable it to play a role in limiting malnourishment and income insecurity in Africa.

The Africa Biosciences Challenge Fund (ABCF) fellowship offered by the BecA-ILRI Hub provided me an opportunity to study this under-researched crop. Using high throughput genotyping technologies, I will generate information that will contribute to breeding initiatives to improve this crop.

With full support from the BecA-ILRI Hub, I also had the opportunity to attend the 13^th Solanaceae Conference at University of California, Davis (UC-Davis) from 12–16 September, 2016. During the meeting themed from advances to applications, I made a one-minute pitch using a poster of my work ‘Generating genomic tools for efficient breeding of African eggplant’.

The career panel workshop chaired by Ann Powell from the UC Davis department of plant sciences afforded me the opportunity to learn from and interact with international professionals from the public sector and industry. I participated in discussions on cutting edge research in genomic tools, advances and applications for the Solanaceae species.

I am grateful for the research, capacity building opportunity and support I have received at the BecA-ILRI Hub. The training and mentorship has greatly increased my capacity in molecular biology, and bioinformatics. I am now also confident in communicating my research with scientific and non scientific audiences

About Fred Masika
Fred Bwayo Masika works with Uganda Christian University in The Department of Agricultural and Biological Sciences. He has a MSc. Botany (Genetics and Molecular biology) from Makerere University.  Realizing that there is narrowing food diversity and recognizing the potential role of traditional vegetables in combating nutrient deficiencies, Masika is passionate about research of underutilized nutritious vegetables such as those of the Solanaceae family. His work towards generating genomic tools in African eggplant will help boost production of African eggplant and related species.

Birindwa Ahadi from Univesité Evangelique en Afrique, DRC working at the BecA-ILRI Hub Laboratory (photo: BecA-ILRI Hub/Sylvia Muthoni)

Small ruminant farming in the Democratic Republic of Congo (DRC) accounts for more than 72 percent of household incomes. However, according FAO reports, this important source of meat, milk, skin and organic manure in DRC is under threat.

An estimated 1,000,000 goats and 600,000 sheep are at risk of contracting peste des petits ruminants (PPR) disease–also referred to as ‘goat plague’ resulting in annual losses of approximately USD 5.3 million.

From December 2015, Birindwa Ahadi, a lecturer at the Univesité Evangelique en Afrique, DRC has been at the Biosciences eastern and central Africa-International Livestock Research Institute (BecA-ILRI) Hub seeking a solution to the challenge facing thousands of smallholder farmers in his country.

Through an Africa Biosciences Challenge Fund (ABCF) fellowship at the BecA-ILRI Hub, Ahadi has been carrying out an in-depth analysis of incidences of the PPR virus in goats and sheep. Ahadi hopes to identify PPR hotspots in DRC and identify PPR risk factors. These findings will contribute to appropriate control strategies and policies to be included in a national program for control and eradication of PPR and other related trans boundary diseases.

‘Being the first published report on the prevalence of PPR in eastern DRC, my research at the BecA-ILRI Hub will make a significant contribution to the Ministry of Agriculture in my country,’ says Ahadi.

Since its inception in 2010, the ABCF program has contributed to strengthening capacities of individual scientists and institutions in sub Saharan Africa and is looking forward to supporting DRC in managing the PPR disease that has a high negative impact on food and economic security for smallholder farmers.

Post-doctoral scientists Gabriela Chavez from Auburn University (left) and William Sharpee (2nd right) from North Carolina State University interact with cassava farmers in the western region of Kenya during a whitefly collecting exercise

Cassava is an important food crop for millions of people in sub-Saharan Africa, but unfortunately this crop is facing a decline in production across the continent due to Cassava Mosaic Disease (CMD). I came to the BecA-ILRI Hub in August to work on a project funded by the National Science Foundation Partnerships for International Research and Education (NSF-PIRE) to analyze the evolution of the viruses that cause this disease.

The purpose of this project is to understand how African cassava mosaic virus (ACMV) and East African cassava mosaic virus (EACMV), the causal agents of CMD, evolve during vegetative propagation of infected cassava plants versus being transmitted via whiteflies. It is our goal to understand how these viruses evolve in order to develop strategies that will hinder the development of more virulent strains and thus prevent future outbreaks of CMD.

When I first arrived in Kenya, I travelled to the shores of Lake Victoria in the western part of Kenya to collect whiteflies for the establishment of a colony at the BecA-ILRI Hub. This was a good opportunity to interact with local farmers and see the devastating effects that this disease has on cassava production in Kenya. Once the individual whiteflies were collected we set up a room dedicated to establishing a colony to be used in future experiments.

Because multiple species of whiteflies exist, my colleagues and I have focused our efforts on understanding the genetic make up of the colony to ensure that we have a single species for our experiments. Additionally, we established procedures for the propagation and growth of cassava in the greenhouse. We continue to lay the groundwork for pilot experiments that will act as the basis for our future work.

I am excited to be a part of this project and the BecA-ILRI Hub community. I am grateful for everyone’s support and input and look forward to great discoveries in the future.

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Read related stories:

International partnership on Cassava virus evolution launched in Africa

 Gates grant boosts fight against cassava disease

Auburn University Post-Doc Tracks Cassava Virus History In East Africa

The BecA-ILRI Hub strengthens partnership with North Carolina State University

Anne Muiruri – APTECA program coordinator at the BecA-ILRI Hub (photo: BecA-ILRI Hub/Alnoor Abdulla)

The Aflatoxin Proficiency Testing and Control in Africa (APTECA) program hosted by the mycotoxin diagnostics platform at the Biosciences eastern and central Africa-International Livestock Research Institute (BecA-ILRI) Hub is contributing to the availability of safe maize on the African market.

The program, managed by the Texas A&M University, USA, was initiated to support the commercial maize milling sector in Kenya through a public-private partnership. Cereal millers which participate in the voluntary program manage aflatoxin risk by improving their quality systems to accurately perform their own tests for aflatoxins in maize flour.

Proficiency testing program

Participation in the APTECA program improves testing accuracy through qualification of the mill’s laboratory analysts; use of working controls with a known level of aflatoxin; routine proficiency testing; and verification of mill results by the ISO accredited Texas A&M AgriLife laboratory housed at the BecA-ILRI Hub.

In 2015, 31 laboratory analysts from commercial mills across Kenya attended training and qualified to analyse maize flour using validated aflatoxin testing platforms. These qualified individuals analyse working control samples twice a week at their respective mills to ensure testing accuracy and results are evaluated using a statistical process control charting technique. Further verification of mills’ aflatoxin test results of finished product occurs at the Texas A&M AgriLife laboratory at the BecA-ILRI Hub and results are sent to the APTECA mills to assist in quality improvement and aflatoxin risk management.

Already, APTECA has hosted five proficiency testing exercises involving 30 industry and public sector laboratories. The companies involved in the project include Osho Grain Millers; Unga Holdings; Alpha Mills; Capwell Industries; Kabansora Millers; Kenblest Group; Maisha Flour Mills; Mombasa Maize Millers; Pembe Flour Mills; Premier Group; and United Millers all from Kenya.

Co-regulation

The APTECA research is part of an effort to explore co-regulation of aflatoxin as a regulatory risk-management policy alternative with the aim of improving food safety and facilitating trade in Africa. Co-regulation involves a government-private sector partnership in regulation that includes statutory or government-backed codes of practice combined with regulatory and industry oversight. A marketing study conducted by the International Food Policy Research Institute (IFPRI) and the Western Michigan University in collaboration with Texas A&M AgriLife explored the impact on sales of marketing products branded with a logo on packages of maize meal stating ‘Aflatoxin Tested Process Verified by APTECA.’ This logo conforms to the East African Community labelling requirements and AgriLife has received trademark approval for it from the Kenya Intellectual Property Institute.

A memorandum of understanding (MoU) with a Kenya regulatory authority, accreditation of the AgriLife laboratory activities by the Kenya Accreditation Service, and training regulatory chemists from six countries and nine agencies has helped lay the groundwork for a regional public-private sector partnership to manage aflatoxin risk and facilitate trade among countries in the Common Market for Eastern and Southern Africa.

The partnership brings together the Kenya Cereal Millers Association—which has over ten million customers, including the urban poor—and the Texas A&M AgriLife laboratory which is hosted at the BecA-ILRI Hub. It is enabling millers to accurately perform their own tests for aflatoxins in maize flour, reducing aflatoxin risk and improving food safety for an estimated 16 million Kenyans.

Aflatoxins are a naturally occurring carcinogenic by-product of common fungi that grow on grains and other food crops, particularly maize and groundnuts. Highly carcinogenic, aflatoxins are lethal in high doses, with chronic exposure potentially stunting infant development, blocking nutrient absorption and suppressing the immune system.

Preventing human exposure to aflatoxins involves removing crops with unacceptable aflatoxin contents from both foods and feeds.

Paloma Fernandes, the chief executive of the Kenya Cereal Millers Association, will give a presentation on industry-led approaches to controlling aflatoxin in the country’s food supply chain at the ‘Strengthening systems to optimize agriculture and nutrition outcomes in Africa’ side event.

Read event concept note: Strengthening Systems to Optimize Agriculture and Nutrition Outcomes in Africa

For more information on the Africa Agriculture Science Week visit: http://faraafrica.org/aasw7/

Follow the event on twitter: #AASW7

Read related articles:

A vision for safe, affordable and adequate food

Providing safe maize for Africa: Aflatoxin Proficiency Testing and Control in Africa project at the BecA-ILRI Hub

Regional Aflatoxin control organization recognizes role of the BecA-ILRI Hub in fighting aflatoxins

Having a milling capacity of 1.6 million tonnes of maize per year and constituting 85 percent of the commercial flour on the shelves sold to about 10 million consumers annually, the Cereal Millers Association (CMA) bears the heavy responsibility of providing safe, affordable and adequate food for their consumers. This responsibility is at the heart of our vision as an association which comprises 27 of the largest millers in the country.

Our four-year relationship with the BecA-ILRI Hub’s aflatoxin research project was borne out of our quest to bridge the existing gap in best practices for diagnosis of aflatoxins at the millers’ level. In efforts to find a solution, we participated in various national forums on the control of aflatoxins in the food value chain in Kenya and eventually made the connection with the project.

Through our collaboration with the BecA-ILRI Hub, CMA staff members have received training on the proper use of aflatoxin diagnostics equipment to get the most accurate results.Visits by the BecA-ILRI Hub scientists, research technicians and project collaborators to three CMA mills has helped us ascertain the levels of testing, training needs and ways in which we can improve our storage, transport and testing facilities.

In order for us to take adequate measures in providing safe food for Kenyans, we have extended
our collaboration to exploratory research on the types of aflatoxins we are dealing with at our mills and will provide samples of both wheat and maize to the BecA-ILRI Hub for analysis.

Through this partnership, we have also identified a consultant from Texas A&M University, USA, to
develop and test the feasibility of maize sampling and aflatoxin testing protocols for use in Kenyan maize mills – an initiative in which many of our mills are involved.

The dream of CMA is to have a fully-fledged laboratory for testing of aflatoxins and we believe with the support of research institutions like the BecA-ILRI Hub, this dream is not very distant. Ultimately we hope that we can achieve our goal to provide safe, affordable and adequate food for all our consumers.